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GC-2spd (ts) was established by stable cotransfection of freshly isolated spermatocytes with the SV40 large T antigen gene (pSV3neo, see ATCC 37150) and a temperature sensitive mutant of the p53 tumor suppressor gene (LTRp53cG9). Cells were selected with G-418, cultivated for 6 months and single cell cloned three times by limiting dilution.

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